ABSTRACT
OBJECTIVE: To explore the hydrolysis kinetics of capilliposide B and capilliposide C under different temperatures and pH values, and identify the hydrolysis products. METHODS: The LC-B and LC-C were quantified by HPLC, then the hydrolysis process was deduced. Samples were taken to investigate the stability of LC-C and LC-B at different time points under the conditions of 30, 50, 70℃, and pH 8.0, 9.0, 10.0, 11.0, respectively. The hydrolysis products were identified by mass spectrometry and nuclear magnetic resonance spectroscopy. RESULTS: The hydrolysis reaction rates of LC-B and LC-C obeyed first-order kinetics under different pH values and temperatures. And they were related to hydrolysis temperature and pH: at the same temperature, the higher the pH, the faster the hydrolysis rate; at the same pH, the higher the temperature, the faster the hydrolysis rate. The hydrolysis product was capilliposide A. CONCLUSION: The study provided an effective method for the preparation of LC-A.
ABSTRACT
<p><b>OBJECTIVE</b>To test the sensitivity and specificity of alpha-fetoprotein (AFP) colloidal gold diagnostic strip as compared with RIA-AFP diagnostic kit.</p><p><b>METHODS</b>Double blind method was used to test sera from 426 healthy people and 1567 patients selected in two general hospitals and a cancer hospital. Each serum was measured by RIA-AFP and AFP colloidal gold diagnostic strip on the same day. RIA-AFP diagnostic results was identified as true positive or negative.</p><p><b>RESULTS</b>Among 426 healthy people, all the RIA-AFP test showed negative result (serum AFP concentration less than 25 IU/ml), but the AFP colloidal gold diagnostic strip had 1.88% false positive. When comparing the result from 1567 patients measured by RIA-AFP, the sensitivity and specificity of AFP colloidal gold diagnostic strip were 99.3%, and 97.2%, respectively. The crude correspondence rate between the two diagnostic regents was 97.6%.</p><p><b>CONCLUSION</b>AFP colloidal gold diagnostic strip showed very good result and could be used as a screening diagnostic kit in clinic and hospital settings.</p>